Knowledge-enriched characterization of cell populations. Morphological information and facts from visuals can include to the richness of flow cytometry facts. Such as, the figure shows an normally conventional apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Every population to expose morphologically distinct functions. These insights could not are actually acquired from multiplex staining by itself.
As samples are obtained to the Attune CytPix Flow Cytometer, the substantial-speed brightfield digital camera captures and outlets photographs of detected events in a level as many as six,000 photos for every 2nd, dependant upon the flow amount and graphic size. For increased flexibility, Attune Cytometric Software program allows you to modify the image capture frequency as essential and choose a certain gate for picture seize, if essential.
(B) Uncooked 264.7 macrophages tend to be annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The volume of singlets accessible for Investigation boosts as soon as the consumer trains and employs a more accurate design.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a significant-speed brightfield digital camera for simultaneous superior throughput flow cytometry and high resolution brightfield imaging.
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Listed here, a seasoned person has gated singlets confidently. Just after assessing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of a lot more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for amplified workflow efficiency, and increase robotic integration for optimum throughput.
Dependable image quality even at higher throughput Acoustic focusing in addition to a significant-velocity digicam Merge to impression these T cells continuously at small or superior flow charges. Simply regulate concentration and digital camera settings to fulfill experimental requirements.
Early indications of this contamination had initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also enables you to use morphological features to discover intriguing subpopulations that will not be obvious from flow cytometry facts by itself.
Users can look at occasion pictures in true-time to substantiate sample top quality, optimize protocols, and record the morphology of each and every mobile inhabitants discovered for Investigation. Imaging capture also Added benefits from get more info acoustic concentrating, which really helps to position the cells to ensure a sharp, centered image is received.
AI-driven automated picture analysis Along with the Attune Cytometric Software translates event functions into unique morphology parameters which can be combined with standard fluorescence and scatter parameters to expose hugely reproducible population adjustments and unique subsets.
Application note: Automated graphic analysis decreases person-to-user variability in flow cytometry gating tactics
Leverage the strength of Superior know-how and innovation to accelerate your discoveries. Trust during the Attune Flow Cytometer loved ones to provide the dependability, velocity, and precision that set your perform aside.
No wash/no lyse leukocyte Evaluation with imaging. Cells were being acquired from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI allows people to coach AI products for expanded apps. (A) The “Prepare Product” aspect supplies annotation equipment and authentic-time model education precision.
Gating on these extended parameters enables you to quickly and accurately identify populations of fascination and make sure gating system with little if any handbook overview. The Attune program features access to state-of-the-art device learning Assessment resources in an expertly made consumer interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer is a higher-parameter spectral analyzer, offering equally spectral unmixing and traditional flow capabilities for in depth and adaptable mobile Examination.
The Attune CytPix Flow Cytometer lets users to visualise samples throughout acquisition, verify the accuracy of gating approaches, and guidance final results with trustworthy documentation for publication functions.
What appeared to be a homogeneous population is in fact a lot more various—an insight that needs to be regarded as when interpreting effects.